KMID : 0358920060330040587
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Journal of the Korean Academy of Pedodontics 2006 Volume.33 No. 4 p.587 ~ p.596
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Generation of a monoclonal antibody against AgI/II, a cellular surface protein of Streptococcus mutans GS5
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Cheon Cheol-Wan
Baik Byeong-Ju Yang Yeon-Mi Han Ji-Hye Kim Jae-Gon
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Abstract
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Most of oral streptococci express the Antigen I/II (AgI/II) proteins, cell wall anchored adhesions. AgI/II protein binds to salivary agglutinin glycoprotein, a component of tooth pellicle and to ligands in other bacteria. These associations play important roles in bacterial colonization. Recently, it was reported that diverse host molecules also interact with AgI/II protein and that these interactions induce inflammatory responses from host cells. Among mutans streptococci containing -type hemolytic activity, Streptococcus mutans is a causative agent for dental caries. Compared with many other strains of S. mutans, GS-5 strain is unique in that this bacterium expresses truncated secretory AgI/II protein due to the nonsense mutation in the agI/II gene. This indicates that S. mutans GS-5 has a different clinical role and a recent report supported this idea based on the results from clinically isolated S. mutans strains. Previously, we had cloned agI/II gene from S. mutans GS-5 and generated recombinant N-terminal AgI/II protein. In this study, we further produced a hybridoma line expressing anti-AgI/II monoclonal antibodies named as 1C11A. This antibody showed high sensitivity to AgI/II protein in Western blot and ELISA. This new reagent will provide a basis for investigating the mechanisms of AgI/II-related diseases.
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KEYWORD
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Streptococcus mutans, Antigen I/II, Monoclonal antibody
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